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- Title:
- Comparative study of micronucleus assay and chromosomal aberration analysis in V79 cells exposed to ethylene oxide
- Author(s):
- Zhong, B; Gu, Z; Whong, W; Wallace, WE; Ong, T
- Address:
- Tong-Man Ong, Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, 944 Chestnut Ridge Road, Morgantown, WV 26505-2888
| NIOSH Office/Division: |
DRDS |
- Source:
- Teratog, Carcinog, Mutagen 1991 Jan; 11(5):227-233
| State/Territory: |
WV |
| Coden: |
TCMUD8 |
| ISSN: |
0270-3211 |
| NIOSHTIC Control Number: |
00207781 |
| Document Type: |
Journal Article |
- NIOSHTIC Descriptor(s):
- NIOSH Author // In vitro studies // Epoxides // Lung cells // Chromosome disorders // Dose response // Clastogens // Cell division // Mammalian cells
- CAS Registry Number(s):
- 75-21-8
- Abstract:
- Induction of micronuclei and chromosome aberrations in hamster lung fibroblasts by ethylene-oxide (75218) was examined. Chinese-hamster- V79 lung fibroblasts were cultured and exposed to 3500, 6900, 13,800, or 27,700 parts per million (ppm) ethylene-oxide for 30 minutes. The cells were examined for chromosome aberrations. Other V79 cell cultures were exposed to 457, 1372, 4115, or 12344ppm ethylene-oxide for 30 minutes. The cells were then treated with 3 micrograms per milliliter cytochalasin-B (CytB). The cells were scored for micronuclei. The proportion of binucleated cells was determined. Ethylene-oxide induced significant dose dependent increases in chromosome aberration frequency. The aberrations consisted of chromatid and isochromatid breaks, fragments, minutes, and exchanges. Ethylene-oxide increased the frequency of micronucleated V79 cells; however, only the increase induced by the 12,344ppm exposure was statistically significant. The increases were approximately 1.5 to 3 times higher in cells that had been treated with CytB. The proportion of binucleated cells was increased by CytB but decreased by ethylene-oxide. The decrease induced by ethylene-oxide was dose dependent. The authors conclude that the chromosome aberration and micronuclei induction data indicate that ethylene-oxide is a clastogen. Chromosome aberration analysis appears to be a more sensitive technique for detecting clastogenicity than micronuclei analysis.
- URL:
- http://dx.doi.org/10.1002/tcm.1770110502
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©2010 Canadian Centre for Occupational Health & Safety 